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gene exp birc3 hs00154109 m1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene exp birc3 hs00154109 m1
    Mean (logRQ ± SE) expression of <t>BIRC3</t> and XAF1 genes depending on PD-1 expression, The U Mann Whitney Test.
    Gene Exp Birc3 Hs00154109 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/birc3/Gene+Exp%2E+BIRC3%2C+Hs00154109_m1/pmc12913816-238-14--1
    Average 91 stars, based on 1 article reviews
    gene exp birc3 hs00154109 m1 - by Bioz Stars, 2026-11
    91/100 stars

    Images

    1) Product Images from "Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas"

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    Journal: Scientific Reports

    doi: 10.1038/s41598-026-35887-7

    Mean (logRQ ± SE) expression of BIRC3 and XAF1 genes depending on PD-1 expression, The U Mann Whitney Test.
    Figure Legend Snippet: Mean (logRQ ± SE) expression of BIRC3 and XAF1 genes depending on PD-1 expression, The U Mann Whitney Test.

    Techniques Used: Expressing, MANN-WHITNEY

    Mean (logRQ ± SE) expression of BIRC2 (p = 0.025) , BIRC3 and BIRC5 genes depending on survival rate (< 20 months and > 20 months), (The U Mann Whitney Test)
    Figure Legend Snippet: Mean (logRQ ± SE) expression of BIRC2 (p = 0.025) , BIRC3 and BIRC5 genes depending on survival rate (< 20 months and > 20 months), (The U Mann Whitney Test)

    Techniques Used: Expressing, MANN-WHITNEY

    Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on survival rate (< 12 months and > 12 months), (The U Mann Whitney Test)
    Figure Legend Snippet: Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on survival rate (< 12 months and > 12 months), (The U Mann Whitney Test)

    Techniques Used: Expressing, MANN-WHITNEY

    Scatterplot between BIRC2 gene expression (logRQ) and survival rate ( r = -0.478 p < 0.05 Spearman’s rank correlations).
    Figure Legend Snippet: Scatterplot between BIRC2 gene expression (logRQ) and survival rate ( r = -0.478 p < 0.05 Spearman’s rank correlations).

    Techniques Used: Gene Expression

    Scatterplot between BIRC3 gene expression (logRQ) and survival rate ( r = -0.536 p < 0.05 Spearman’s rank correlations).
    Figure Legend Snippet: Scatterplot between BIRC3 gene expression (logRQ) and survival rate ( r = -0.536 p < 0.05 Spearman’s rank correlations).

    Techniques Used: Gene Expression

    Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression free survival (< 20 months and > 20 months), (The U Mann Whitney Test)
    Figure Legend Snippet: Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression free survival (< 20 months and > 20 months), (The U Mann Whitney Test)

    Techniques Used: Expressing, MANN-WHITNEY

    Scatterplot between BIRC2 gene expression (logRQ) and progression-free survival ( r =-0.481 p < 0.05 Spearman’s rank correlations).
    Figure Legend Snippet: Scatterplot between BIRC2 gene expression (logRQ) and progression-free survival ( r =-0.481 p < 0.05 Spearman’s rank correlations).

    Techniques Used: Gene Expression

    Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression-free survival (< 12 months and > 12 months), (The U Mann Whitney Test)
    Figure Legend Snippet: Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression-free survival (< 12 months and > 12 months), (The U Mann Whitney Test)

    Techniques Used: Expressing, MANN-WHITNEY

    Scatterplot between BIRC3 gene expression (logRQ) and progression-free survival ( r =-0.536 p < 0.05 Spearman’s rank correlations).
    Figure Legend Snippet: Scatterplot between BIRC3 gene expression (logRQ) and progression-free survival ( r =-0.536 p < 0.05 Spearman’s rank correlations).

    Techniques Used: Gene Expression

    Forest plot of odds ratios (OR) from univariate logistic regression for dichotomized survival endpoints (OS/PFS) in the intersection set, using BIRC2 expression (logRQ) as predictor.
    Figure Legend Snippet: Forest plot of odds ratios (OR) from univariate logistic regression for dichotomized survival endpoints (OS/PFS) in the intersection set, using BIRC2 expression (logRQ) as predictor.

    Techniques Used: Expressing

    Forest plot of odds ratios (OR) from univariate logistic regression for dichotomized survival endpoints (OS/PFS) in the intersection set, using BIRC3 expression (logRQ) as predictor.
    Figure Legend Snippet: Forest plot of odds ratios (OR) from univariate logistic regression for dichotomized survival endpoints (OS/PFS) in the intersection set, using BIRC3 expression (logRQ) as predictor.

    Techniques Used: Expressing

    Kaplan–Meier overall survival stratified by BIRC2 expression group (high vs. low). Log-rank p = 0.023* BIRC2 group (high = logRQ BIRC2 >-0.077794 (median) /low = logRQ BIRC2 <=-0.077794).
    Figure Legend Snippet: Kaplan–Meier overall survival stratified by BIRC2 expression group (high vs. low). Log-rank p = 0.023* BIRC2 group (high = logRQ BIRC2 >-0.077794 (median) /low = logRQ BIRC2 <=-0.077794).

    Techniques Used: Expressing

    Kaplan–Meier overall survival stratified by BIRC3 expression group (high vs. low). Log-rank p = 0.1824. BIRC3 group (high = logRQ BIRC3 >-0.316210 (median) /low = logRQ BIRC3 <=-0.316210).
    Figure Legend Snippet: Kaplan–Meier overall survival stratified by BIRC3 expression group (high vs. low). Log-rank p = 0.1824. BIRC3 group (high = logRQ BIRC3 >-0.316210 (median) /low = logRQ BIRC3 <=-0.316210).

    Techniques Used: Expressing

    Related Articles

    Immunofluorescence:

    Article Title: Endothelial Birc3 promotes renal fibrosis through modulating Drp1-mediated mitochondrial fission via MAPK/PI3K/Akt pathway.
    Article Snippet: Images were taken with the confocal laser microscope (FV1000; Olympus, Tokyo, Japan) and quantified through Image J software. .. Regarding immunofluorescence staining, the renal tissue sections underwent overnight incubation at 4 ◦C with the primary antibodies such as Birc3 (1:300, PA5-51700, Invitrogen), CD31 (1:500, ab264486, Abcam). .. On the second day, Goat Anti-Rat IgG H&L (Alexa Fluor® 647), Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (1:400, ab307262, ab150077, Abcam) secondary antibodies were employed.

    Staining:

    Article Title: Endothelial Birc3 promotes renal fibrosis through modulating Drp1-mediated mitochondrial fission via MAPK/PI3K/Akt pathway.
    Article Snippet: Images were taken with the confocal laser microscope (FV1000; Olympus, Tokyo, Japan) and quantified through Image J software. .. Regarding immunofluorescence staining, the renal tissue sections underwent overnight incubation at 4 ◦C with the primary antibodies such as Birc3 (1:300, PA5-51700, Invitrogen), CD31 (1:500, ab264486, Abcam). .. On the second day, Goat Anti-Rat IgG H&L (Alexa Fluor® 647), Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (1:400, ab307262, ab150077, Abcam) secondary antibodies were employed.

    Article Title: Endothelial Birc3 promotes renal fibrosis through modulating Drp1-mediated mitochondrial fission via MAPK/PI3K/Akt pathway.
    Article Snippet: .. Immunohistochemical staining involved identifying and analyzing target proteins by incubating kidney sections with primary antibodies such as Birc3 (1:300, PA5-51700, Invitrogen), CD31 (1:500, #77699, CST), Col1a1 (1:500, A1352, Abclonal), followed by a secondary antibody. ..

    Incubation:

    Article Title: Endothelial Birc3 promotes renal fibrosis through modulating Drp1-mediated mitochondrial fission via MAPK/PI3K/Akt pathway.
    Article Snippet: Images were taken with the confocal laser microscope (FV1000; Olympus, Tokyo, Japan) and quantified through Image J software. .. Regarding immunofluorescence staining, the renal tissue sections underwent overnight incubation at 4 ◦C with the primary antibodies such as Birc3 (1:300, PA5-51700, Invitrogen), CD31 (1:500, ab264486, Abcam). .. On the second day, Goat Anti-Rat IgG H&L (Alexa Fluor® 647), Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (1:400, ab307262, ab150077, Abcam) secondary antibodies were employed.

    Polymerase Chain Reaction:

    Article Title: Leveraging Automation toward Development of a High-Throughput Gene Expression Profiling Platform.
    Article Snippet: We previously developed a panel of one-step real-time quantitative reverse transcription PCR (one-step qRT-PCR; hereafter referred to as qRT-PCR) assays to assess compound efficacy.. However, these high-cost, conventional qRT-PCR manual assays are not amenable to high-throughput screen (HTS) analysis in a time-sensitive and complex drug discovery process.. Here, we report the establishment of an automated gene expression platform using in-house lysis conditions that allows the study of various cell lines, including primary T cells.

    Immunohistochemical staining:

    Article Title: Endothelial Birc3 promotes renal fibrosis through modulating Drp1-mediated mitochondrial fission via MAPK/PI3K/Akt pathway.
    Article Snippet: .. Immunohistochemical staining involved identifying and analyzing target proteins by incubating kidney sections with primary antibodies such as Birc3 (1:300, PA5-51700, Invitrogen), CD31 (1:500, #77699, CST), Col1a1 (1:500, A1352, Abclonal), followed by a secondary antibody. ..



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    Image Search Results


    Mean (logRQ ± SE) expression of BIRC3 and XAF1 genes depending on PD-1 expression, The U Mann Whitney Test.

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC3 and XAF1 genes depending on PD-1 expression, The U Mann Whitney Test.

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Expressing, MANN-WHITNEY

    Mean (logRQ ± SE) expression of BIRC2 (p = 0.025) , BIRC3 and BIRC5 genes depending on survival rate (< 20 months and > 20 months), (The U Mann Whitney Test)

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC2 (p = 0.025) , BIRC3 and BIRC5 genes depending on survival rate (< 20 months and > 20 months), (The U Mann Whitney Test)

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Expressing, MANN-WHITNEY

    Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on survival rate (< 12 months and > 12 months), (The U Mann Whitney Test)

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on survival rate (< 12 months and > 12 months), (The U Mann Whitney Test)

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Expressing, MANN-WHITNEY

    Scatterplot between BIRC2 gene expression (logRQ) and survival rate ( r = -0.478 p < 0.05 Spearman’s rank correlations).

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Scatterplot between BIRC2 gene expression (logRQ) and survival rate ( r = -0.478 p < 0.05 Spearman’s rank correlations).

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Gene Expression

    Scatterplot between BIRC3 gene expression (logRQ) and survival rate ( r = -0.536 p < 0.05 Spearman’s rank correlations).

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Scatterplot between BIRC3 gene expression (logRQ) and survival rate ( r = -0.536 p < 0.05 Spearman’s rank correlations).

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Gene Expression

    Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression free survival (< 20 months and > 20 months), (The U Mann Whitney Test)

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression free survival (< 20 months and > 20 months), (The U Mann Whitney Test)

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Expressing, MANN-WHITNEY

    Scatterplot between BIRC2 gene expression (logRQ) and progression-free survival ( r =-0.481 p < 0.05 Spearman’s rank correlations).

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Scatterplot between BIRC2 gene expression (logRQ) and progression-free survival ( r =-0.481 p < 0.05 Spearman’s rank correlations).

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Gene Expression

    Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression-free survival (< 12 months and > 12 months), (The U Mann Whitney Test)

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression-free survival (< 12 months and > 12 months), (The U Mann Whitney Test)

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Expressing, MANN-WHITNEY

    Scatterplot between BIRC3 gene expression (logRQ) and progression-free survival ( r =-0.536 p < 0.05 Spearman’s rank correlations).

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Scatterplot between BIRC3 gene expression (logRQ) and progression-free survival ( r =-0.536 p < 0.05 Spearman’s rank correlations).

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Gene Expression

    Forest plot of odds ratios (OR) from univariate logistic regression for dichotomized survival endpoints (OS/PFS) in the intersection set, using BIRC2 expression (logRQ) as predictor.

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Forest plot of odds ratios (OR) from univariate logistic regression for dichotomized survival endpoints (OS/PFS) in the intersection set, using BIRC2 expression (logRQ) as predictor.

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Expressing

    Forest plot of odds ratios (OR) from univariate logistic regression for dichotomized survival endpoints (OS/PFS) in the intersection set, using BIRC3 expression (logRQ) as predictor.

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Forest plot of odds ratios (OR) from univariate logistic regression for dichotomized survival endpoints (OS/PFS) in the intersection set, using BIRC3 expression (logRQ) as predictor.

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Expressing

    Kaplan–Meier overall survival stratified by BIRC2 expression group (high vs. low). Log-rank p = 0.023* BIRC2 group (high = logRQ BIRC2 >-0.077794 (median) /low = logRQ BIRC2 <=-0.077794).

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Kaplan–Meier overall survival stratified by BIRC2 expression group (high vs. low). Log-rank p = 0.023* BIRC2 group (high = logRQ BIRC2 >-0.077794 (median) /low = logRQ BIRC2 <=-0.077794).

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Expressing

    Kaplan–Meier overall survival stratified by BIRC3 expression group (high vs. low). Log-rank p = 0.1824. BIRC3 group (high = logRQ BIRC3 >-0.316210 (median) /low = logRQ BIRC3 <=-0.316210).

    Journal: Scientific Reports

    Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

    doi: 10.1038/s41598-026-35887-7

    Figure Lengend Snippet: Kaplan–Meier overall survival stratified by BIRC3 expression group (high vs. low). Log-rank p = 0.1824. BIRC3 group (high = logRQ BIRC3 >-0.316210 (median) /low = logRQ BIRC3 <=-0.316210).

    Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

    Techniques: Expressing

    Examples of PR regulated proteins and pathways involved in cell proliferation and apoptosis. The quantitative proteomic analysis identified significant changes of thousands of proteins and many hundreds of phosphorylated peptides in response to PR agonist. This figure shows proteins and pathways that were analyzed and interpreted in greater details. (A) Proteins and complexes involved in R5020 induced G0/G1 and G2/M cell cycle arrest, which could also be triggers for apoptosis. (B) PR regulated mitochondrial proteins involved in R5020 induced apoptosis. Significant increases of HIF1A and its downstream targets such as BNIP3 and NIX activated mitophagy together with upregulated LC3. Prolonged increase of BNIP3 and NIX causes mitochondria dysfunction and loss of mitochondria membrane potential. BNIP3 and NIX can sequester anti-apoptotic proteins such as BCL-2 and BCL-XL. This relieved BH3-only proteins, which facilitated formation of BAX and BAK pores on mitochondria outer membrane, leading to MOMPs, cytochrome c release and CASP9 activation, along with upregulation and releases of pro-apoptotic factors such as AIF, ENDOG, HtrA2/Omi, and Smac/DIABLO. PR induced apoptotic mechanism may also involve some of the 200 mitochondrial proteins regulated by R5020. (C) p53 pathway may also participate in R5020-induced cell apoptosis through upregulation of proteins such as death receptors FAS, DR4 and DR5. Upregulation of MDM2 is involved in a negative feedback regulation of p53. (D) TNF signaling via NFKB is generally pro-survival. It was found to be markedly upregulated. Gene silencing of its key players BIRC3 and TNFAIP3 alone or in combination had no influence on R5020 induced downregulation of effector caspases and apoptosis. (E) True to the holistic nature of proteomic analysis, the study also detected the previously reported upregulation of protooncogenic proteins by R5020, but the upregulation of these proteins are futile and should not be interpreted as evidence for pro-tumoral activity of PR. Additionally, all effector CASPs detected in the MS data and in immunoblots were downregulation despite R5020 induced apoptosis. *Although CASP6 is marginally increased in MS data and immunoblot, its activation by cleavage is evidently reduced in immunoblot. Blue downward arrow = Downregulation; Red upward arrow = Upregulation.

    Journal: Scientific Reports

    Article Title: Proteomics analysis reveals progesterone receptor induced mitochondria-mediated apoptosis in breast cancer cells

    doi: 10.1038/s41598-025-28183-3

    Figure Lengend Snippet: Examples of PR regulated proteins and pathways involved in cell proliferation and apoptosis. The quantitative proteomic analysis identified significant changes of thousands of proteins and many hundreds of phosphorylated peptides in response to PR agonist. This figure shows proteins and pathways that were analyzed and interpreted in greater details. (A) Proteins and complexes involved in R5020 induced G0/G1 and G2/M cell cycle arrest, which could also be triggers for apoptosis. (B) PR regulated mitochondrial proteins involved in R5020 induced apoptosis. Significant increases of HIF1A and its downstream targets such as BNIP3 and NIX activated mitophagy together with upregulated LC3. Prolonged increase of BNIP3 and NIX causes mitochondria dysfunction and loss of mitochondria membrane potential. BNIP3 and NIX can sequester anti-apoptotic proteins such as BCL-2 and BCL-XL. This relieved BH3-only proteins, which facilitated formation of BAX and BAK pores on mitochondria outer membrane, leading to MOMPs, cytochrome c release and CASP9 activation, along with upregulation and releases of pro-apoptotic factors such as AIF, ENDOG, HtrA2/Omi, and Smac/DIABLO. PR induced apoptotic mechanism may also involve some of the 200 mitochondrial proteins regulated by R5020. (C) p53 pathway may also participate in R5020-induced cell apoptosis through upregulation of proteins such as death receptors FAS, DR4 and DR5. Upregulation of MDM2 is involved in a negative feedback regulation of p53. (D) TNF signaling via NFKB is generally pro-survival. It was found to be markedly upregulated. Gene silencing of its key players BIRC3 and TNFAIP3 alone or in combination had no influence on R5020 induced downregulation of effector caspases and apoptosis. (E) True to the holistic nature of proteomic analysis, the study also detected the previously reported upregulation of protooncogenic proteins by R5020, but the upregulation of these proteins are futile and should not be interpreted as evidence for pro-tumoral activity of PR. Additionally, all effector CASPs detected in the MS data and in immunoblots were downregulation despite R5020 induced apoptosis. *Although CASP6 is marginally increased in MS data and immunoblot, its activation by cleavage is evidently reduced in immunoblot. Blue downward arrow = Downregulation; Red upward arrow = Upregulation.

    Article Snippet: BIRC3 , 1:1000 , Cell Signaling Technology, Danvers, MA, USA , 3130.

    Techniques: Membrane, Activation Assay, Activity Assay, Western Blot

    Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

    Journal: BMC Cancer

    Article Title: Chidamide impedes glycolysis but increases ferroptosis and cisplatin sensitivity of lung cancer cells through downregulating USP35

    doi: 10.1186/s12885-025-14925-z

    Figure Lengend Snippet: Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

    Article Snippet: The primary antibodies against USP35 (proteintech, Wuhan, China, 24559-1-AP), PKM2 (proteintech, 15822-1-AP), FPN (proteintech, 26601-1-AP), BIRC3 (proteintech, 24304-1-AP), β-actin (proteintech, 20536-1-AP) were incubated overnight at 4°C.

    Techniques: Transfection